Fig 5.
Antibody responses of HSV-2-infected guinea pigs vaccinated with GEN-003 or GEN-003/MM-2 vaccine. Guinea pigs were infected intravaginally with 5 × 105 PFU of HSV-2 strain MS, allowed to recover from primary disease, and then vaccinated s.c. three times on days 14, 21, and 36 postinfection with PBS, 15 μg of each antigen only (GEN-003), or 15 μg of each antigen plus 50 μg MM-2 adjuvant (GEN-003/MM-2). (A) Antibody titers in plasma samples (n = 4) to ICP4383-766 or gD2ΔTMR were measured by endpoint ELISA at day 42 postinfection (six days after the third immunization). Data are shown as the mean ELISA endpoint titer of each group ± SEM. (B) Neutralizing antibody titers were determined by a colorimetric assay as described in Materials and Methods. The titer was defined as the reciprocal of the serum dilution required to decrease the OD590 obtained using the virus control by 50%. Data are shown as the mean neutralizing antibody titer of each group ± SEM. P values compare the GEN-003-immunized group to the GEN-003/MM-2-immunized group or to the PBS control group using Student's t test. (C) Antibody titers in plasma samples (n = 4) to ICP4383-766 or gD2ΔTMR were measured by endpoint ELISA at day 42 postinfection (six days after the third immunization). P values compare the GEN-003/MM-2-vaccinated group to the MM-2 control group using Student's t test.