Fig 2.

Identification of highly active IκBα and IκBε siRNAs. (A) Determining the knockdown activity of seven siRNAs targeting IκBα mRNA by qRT-PCR. The location of the siRNA binding sites in the IκBα mRNA is indicated in the schematic diagram at the top of the figure. Gray regions within the coding sequence (flanked by the nucleotide residues) of both mRNAs represent ANK repeats. IκBα siRNA 7 had the best knockdown effect in U1 latently infected cells. It was used in subsequent experiments. (B) Quantification of knockdown by qRT-PCR of 12 siRNAs targeting IκBε mRNA. The location of the siRNA binding sites in the IκBε mRNA is indicated in the schematic diagram at the top of the figure. Gray regions within the coding sequence (flanked by the nucleotide residues) of both mRNAs represent ANK repeats. IκBε siRNA 9 had the best knockdown effect in U1 latently infected cells and was used in subsequent experiments. Results are the means ± standard deviations (SD) from three independent experiments.