Fig 5.

Viral activation using different IκBα and IκBε siRNAs. (A) Effects of different IκBα siRNAs on IκBα knockdown and HIV activation. The locations on the mRNA targeted by seven different IκBα siRNAs are shown in the schematic at the top of the panel. The bar graph shows simultaneously the knockdown effect (white bars) (Fig. 2) of the siRNAs, assayed by qRT-PCR, and the fold increase in HIV p24 antigen produced by each siRNA assessed using a p24 ELISA (black bars). (B) The panel shows the p24 fold increase as a function of IκBα knockdown for the different siRNAs. Spearman's correlation was calculated (ρ = 0.96). (C) Effects of different IκBε siRNAs on IκBε knockdown and HIV activation. The locations on the mRNA targeted by 12 different IκBε siRNAs are shown in the schematic at the top of the panel. The bar graph shows simultaneously the knockdown effect (white bars) (Fig. 2) of the siRNAs, assayed by qRT-PCR, and the fold increase in HIV p24 antigen produced by each siRNA assessed using a p24 ELISA (black bars). (D) The panel shows the p24 fold increase as a function of IκBε knockdown for the different siRNAs. Spearman's correlation was calculated (ρ = 0.93). For both IκBα and IκBε siRNAs, the extent of HIV activation correlated with the extent of IκB knockdown. In the schematic diagrams, gray regions within the coding sequence (flanked by the nucleotide residues) of both mRNAs represent ANK repeats. Results are means ± SD from three independent experiments.