Fig 4.

NEDA is triggered in response to translation in between 5 to 6 h of infection. (A) Cells infected with HSV-1 wt for 4 to 8 h or the HSV-1 ΔPP1α virus for 8 h were analyzed for LC3a signal intensity by flow cytometry. Mock-infected and HSV-1 ΔPP1α virus-infected cells served as negative controls. (B and C) Macrophages were infected with HSV-1 wt and treated with dimethyl sulfoxide (DMSO, control) or 1 μM hippuristanol (hipp) for 1 h. NEDA induction was analyzed at 6 or 8 hpi in immunofluorescence with an antibody against LC3a. The occurrence of an LC3a nuclear rim was analyzed in about 50 cells per condition. Error bars, SD.