Fig 6.
Selective loss of KSHV DNA from BCLB1 cells. (A) DNA from BCLB1 cells treated with shCtrl or shTim for 48 or 72 h was isolated by a total genomic DNA isolation procedure, followed by digestion with BfaI, and then analyzed by ethidium bromide staining after agarose gel electrophoresis (right). M, molecular weight markers. (B) A Southern blot of the same gel as in panel A was probed with TR (top), KSHV 95000 (middle), or cellular α-satellite repeat (bottom) DNA. (C) The KSHV genome copy number was determined from DNA isolated from BCBL1 cells after infection with shCtrl or shTim for 48 or 72 h and assayed by real-time PCR with primers specific for the TR or 95000 region relative to β-actin DNA using the ΔCt method. The error bars indicate standard deviations.