Fig 3.

The impact on replication capacity (RC) of CTL escape mutation in HIV-1 Gag is not correlated with position or genetic conservation (Shannon entropy). (A) The mean replication capacity (RC; dots) with 95% confidence interval (CI; whiskers) is shown for the benchmark RT M184V mutation and for the 29 individual HIV-1B Gag CTL escape mutations in order of their linear position in the Gag polyprotein. For all mutations, the susceptible residue is listed first and the escape residue is listed last. The horizontal dashed line denotes RC_susceptible, which is equal to 1.0 by definition. (B) The CTL escape mutations are plotted from greatest to least reduction in replication (lowest to highest RC value) (white bars) opposite the Shannon entropy of the mutant position (gray bars). (C and D) The lack of correlation between Shannon entropy and RC is shown for all mutations tested (r = 0.16, P = 0.39; Pearson product moment test) (C) and for only those mutations that caused a statistically significant impact on RC (r = 0.27, P = 0.37; Pearson product moment test) (D).