Fig 4.

Host DNA replication factors are associated with the viral replication centers. (A) Immunofluorescence staining of cellular DNA replication factors. At 18 h p.i., mock- and MVC-infected cells were fixed and stained with the indicated antibodies and DAPI. For RFC1 staining, infected cells were also analyzed at 12 h and 24 h p.i. (B) Western blot analysis of cellular DNA replication factors. Mock- and MVC-infected cells were either treated with ATM inhibitor KU55933 (ATMi) or DMSO control. At the indicated times p.i., the cells were collected and analyzed by Western blotting using antibodies against proteins as indicated.