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Brd4 and HPV16 E2 colocalize in punctate nuclear foci only in the presence of HPV16 genome. C33A cells were cotransfected with pUC19-HPV16, GFP-16E2, and HA-16E1. The pUC19-HPV16 plasmid was replaced by pUC19 in the “without-genome” transfection control. At 48-h p.t., cells were fixed and stained using anti-Brd4 antibody and counterstained with DAPI. This experiment was repeated at least 3 times. Bars, 5 μm.
