Fig 2.

Inositol phosphate turnover in transfected or MCMV-infected cells. (A) COS7 cells transfected 24 h previously with constructs expressing either wt US28 (black), US28ΔC54 (gray), US28R129Q (stippled), US28RQ/ΔC54 (striped), US28ΔN[2–16] (checked), or pcDNA3 (white). (B) MEF infected (MOI = 3) 24 h previously with MCMV recombinants expressing either wt or mutated US28 (shading as in panel A) or an M33-null mutant (white). In both panels, cells were incubated in the presence or absence of 10 nM human recombinant CX3CL1, prior to determination of accumulation of phosphorylated [³H]inositol according to methods described previously (28). Data are presented as means ± SD from 2 experiments performed in triplicate; asterisks indicate significant difference between the responses with and without fractalkine (Student's t test; *, P < 0.05; **, P < 0.01; ***, P < 0.001).