Fig 4.

Predosing effects in different mouse strains. (A and B) BALB/c (A) or C57BL/6 (B) mice were predosed i.v. with PBS or 100 μg of poly(I) and were injected 5 min later with 1 × 10¹⁰ vp of Ad5 or Ad5/6. (C) C57BL/6 mice were predosed i.v. with PBS or 3 × 10¹⁰ vp of an irrelevant Ad5-DsRed vector and were then injected i.v. 4 h later with 1 × 10¹⁰ vp of Ad5 or Ad5/6. (D) M-CSF knockout (KO) mice were injected i.v. with 1 × 10¹⁰ vp of Ad5 or Ad5/6. All injected mice were imaged for luciferase expression after 24 h (n, 2 to 5). *, P < 0.05; ns, not significant.