Fig 2.

Infectivity of wild-type and mutant SIVmac239. (A and B) VSV-G-pseudotyped recombinant SIVmac239 viruses were produced in transfected HEK293T cells, and reverse transcriptase activity was measured. Increasing amounts of the WT, A87E, A87D, and A87K viruses, which express GFP, were used to infect Cf2Th (A) or HeLa (B) cells. The percentage of infected, GFP-positive cells was assayed at 48 h postinfection by FACS analysis. (B) Aphidicolin (2 μg/ml) was added to one set of the HeLa target cells (+aphid). The results shown are typical of those obtained in 3 independent experiments. (C) HeLa cells were infected with identical amounts (in RT units) of VSV-G-pseudotyped GFP-expressing recombinant WT and A87E SIV and, as a control, MLV. In some cases, 2 μg/ml aphidicolin was added to the HeLa cells. GFP-positive cells were measured by FACS analysis 48 h after infection. The means and standard deviations of the results obtained in 3 independent experiments are shown.