Fig 5.

Production of 2-LTR circles in infected cells. Recombinant viruses were used to infect HeLa cells, and at the indicated times, the levels of 2-LTR circles were measured by real-time PCR. Both a standard probe (2-LTR), which detects all 2-LTR circles, and a probe (JNCT) specific for canonical 2-LTR junctions were used. Some infected cells were treated with 1 μM raltegravir (Ral). The results for infections by wild-type (WT) SIVmac239 (A) and the A87E (B) and A87D (C) mutants are shown. The values shown represent the percentages of the maximum wild-type SIVmac239 value (823 copies/100 ng DNA at 24 h postinfection after raltegravir treatment). The results shown represent the averages and standard deviations derived from three independent infections.