Fig 6.

Size analysis of 2-LTR products. HeLa cells were infected by recombinant wild-type (WT) and A87E and A87D mutant SIVmac239. At the indicated times after infection, 2-LTR circles were amplified by qPCR using the standard 2-LTR primer set. The products of the qPCRs were run on a 2% agarose gel and stained with ethidium bromide. For some of the experiments, the viruses were heated at 56°C for 1 h, and the cells were treated with 100 mM AZT. In another set of experiments, cells were treated with 1 μM raltegravir. The boxes indicate the material excised and sequenced and are denoted (from left to right and top to bottom) as follows: WT 6-h background, WT 12-h smear, WT 24-h smear and ∼210 bp (within smear), WT 48-h smear, WT plus raltegravir at 12 h and 24 h and ∼210 bp, A87E 6-h background, A87E 12-h smear and ∼210 bp, A87E 24-h smear and ∼210 bp, A87E 48-h smear, A87E 6-h heat-inactivated background, A87E plus raltegravir at 12 h and 24 h and ∼210 bp, A87D 12-h smear, A87D 24-h smear, A87D 48-h smear, A87D 24-h heat-inactivated background, and A87D plus raltegravir at 12 h and 24 h and ∼210 bp.