Fig 8.

Frequency of production of canonical 2-LTR junctions. For each time point following infection of HeLa cells by wild-type (WT) and mutant SIVmac239, the total number of 2-LTR circles was measured by real-time qPCR with a standard 2-LTR probe (see the Fig. 5 legend). In parallel, the number of 2-LTR circles with canonical 2-LTR circle junctions was measured with a specific junctional probe. The number of canonical 2-LTR junctions is shown as a percentage of the total 2-LTR circle junctions observed, with or without raltegravir treatment of the target cells. The asterisks indicate values less than 1%.