Fig 4.
Viral load in blood and organs of primed mice depleted of innate and/or T cell subsets and challenged with ECTV-WT. Groups of 5 female B6 mice immunized with ECTV-TKΔ 35 days previously were left untreated (None) or treated with MAb to deplete triple innate cells (NK cells, granulocytes, and pDC), CD4 plus CD8 T cells, triple innate plus CD4 T cells, triple innate plus CD8 T cells, or triple innate plus CD4 plus CD8 T cells and challenged with 103 PFU of ECTV-WT. Viral load in blood samples (A) was measured by qRT-PCR and presented as means ± standard deviations (SD) of log10 viral genome copy numbers. The dotted line represents the limit of detection of assay. Viral loads in spleen (B) and liver (C) at day 8 p.c. are presented as means ± SD of log10 PFU/g tissue. **, P ≤ 0.01; ***, P ≤ 0.001 (Mann-Whitney nonparametric test). Data shown are from one of two independent experiments with similar results.