Fig 3.

Overexpression of S3D phosphomimetic cofilin interferes with the ability of US3 to cause cell rounding and cell projections. (A and B) ST cells were transfected with US3 encoding plasmid or cotransfected with plasmids encoding US3 and GFP-tagged WT cofilin, S3A cofilin, or S3D cofilin. At 24 h posttransfection, cells were fixed and stained for US3 and nuclei and analyzed for expression of cofilin (GFP; green) and US3 (red). Panel A shows the percentage of transfected cells displaying actin rearrangements, as assessed by cell rounding and the formation of cell projections (means + standard errors of the means; data from three independent experiments), with * indicating P values of <0.05 and ** indicating P values of <0.01. Small blue dots in panel B represent leftover plasmid DNA-containing transfection reagent in cells and on the cover glass.