Fig 1.

Ectopic expression of BPLF1 decreases NF-κB-dependent promoter activity in cells latently infected with EBV. Latently infected B95-8, AGS-EBV, and 293-EBVwt cells were transfected with the NF-κB-Fluc reporter plasmid (0.2 μg/well), along with the pCMV-Rluc plasmid (0.02 μg/well) and either pBPLF1wt or pBPLF1C61A (0.1 μg/well), in 24-well plates. Luciferase assays were performed at 24 hpt. Firefly luciferase activity was normalized to Renilla reniformis luciferase, and the value obtained by transfecting an empty-vector control was set to 100%. Data are shown as means ± SD of the results of 3 biological replicates. **, P < 0.001; *, P < 0.005. Sample lysates were subsequently subjected to immunoblotting with the specific antibodies indicated, and representative results are presented below the graph. In addition, sample lysates of cells transfected with BZLF1 were also included as controls for lytic replication.