Fig 4.

BPLF1 suppresses expression of NF-κB-regulated genes during the EBV lytic life cycle. (A) 293-EBVwt and 293-EBVΔ cells were transfected with control or BZLF1 expression plasmids. At 24 hpi, cells were subjected to qrt-PCR to measure the mRNA levels of NF-κB-dependent genes. Values were normalized to GAPDH mRNA, and the value obtained by transfecting an empty-plasmid control into 293-EBVwt or 293-EBVΔ was set to 1. Data are shown as means ± SD of the results of 3 biological replicates. ***, P < 0.001; **, P < 0.005; *, P < 0.05. (B) RT-PCR was carried out in order to detect BPLF1 mRNA in the same samples described for panel A, followed by an agarose electrophoresis.