Fig 5.

Assembly properties of S57K virus. BHK cells were electroporated with WT or mutant viral RNA and incubated at 37°C for 6 h. The cells were then pulse-labeled for 30 min with [³⁵S]methionine-cysteine and chased for the indicated times at 37°C. (A) The cell lysates and media were immunoprecipitated with a polyclonal antibody to the envelope glycoproteins and analyzed by SDS-PAGE. (B) After immunoprecipitation, an aliquot of the lysates was digested with endoglycosidase H as indicated. p62′, E2′, and E1′ indicate the positions of the protein after Endo H digestion. Data represent the results of 2 independent experiments.