Fig 7.

p62 processing of S57K revertants. BHK cells were infected with WT or mutant virus at a multiplicity of infection of 1 PFU/cell for 90 min at 37°C. After a 5-h incubation, cells were pulse-labeled for 30 min with [³⁵S]methionine-cysteine and chased for 0 (A) or 1 (B) h. Cell lysates were immunoprecipitated with a polyclonal antibody to the envelope glycoproteins and analyzed by SDS-PAGE. Revertants are referred to by the amino acid in the wt SFV sequence, its position, and the revertant amino acid replacement. Data represent the results of 2 independent experiments.