Fig 4.

Impaired levels of cytokines in the sera from WNV-infected ASC^−/− mice. Sera were collected from mock (M)- and WNV-infected WT and ASC^−/− mice (100 PFU via the footpad) at days 2, 4, and 6 after infection. Levels of cytokines IL-1β, IL-6, and IFN-γ (A to C) and chemokines CCL2, CCL5, and CXCL1 (D to F) were measured using a multiplex Luminex assay and expressed as the mean concentrations (pg/ml) ± standard errors of the mean (SEM) and are representative of two independent experiments (n = 7 per group). *, P < 0.05; **, P < 0.001.