Fig 6.

MCM8 and MCM9 associate with RAD51 and are required for RAD51 recruitment to damage sites. (A) MCM9 interacts with RAD51. Endogenous MCM9 protein is immunoprecipitated from U2OS cells and immunoblotted for RAD51 or MCM9 as indicated. EtBr was added to the immunoprecipitation (IP) reaction to exclude the possibility of DNA-dependent interaction. (B to D) ChIP results represent the relative enrichment of RAD51 (B), MCM8 (C), or MCM9 (D) at DSBs (F1-R1) relative to the distant site (F2-R2) at the indicated time points following the expression of I-SceI. We used either control cells or cells depleted of the indicated proteins by siRNA for 24 h. Values represent the means ± the SD of triplicate measurements.