Fig 5.

SIRT1 associates with the SAGA complex. (A) (Top) The indicated FLAG-HA-tagged proteins were isolated from HEK 293T cells, and Dub activity was analyzed by reactions with UbVS; (bottom) samples were prepared as described above, but wild-type SIRT1 samples were diluted as indicated in assay buffer before adding UbVS. (B) FLAG-HA-tagged proteins were isolated from HEK 293T cells and immunoblotted for the indicated proteins. (C) Control IgG or SIRT1-specific antibodies were used to IP endogenous SIRT1 from DSP-cross-linked HEK 293T cells. Immunoblots were probed with the indicated antibodies.