Fig 1.

Regulation of DHX33 during oncogenic stress. (A) Wild-type MEFs were infected with retroviruses encoding Ras^V12, p19^ARF, or empty vector, whole-cell lysates were prepared at 2 days postinfection and were subjected to Western blot analysis with the indicated antibodies. (B) The above-mentioned cells were harvested 3 days postinfection, and whole-cell extracts were subjected to Western blot analysis with the indicated antibodies. (C) Wild-type MEFs were infected with retroviruses encoding Ras^V12 or empty vector, whole-cell extracts were prepared 5 days postinfection and were subjected to Western blot analysis with the indicated antibodies. (D) Arf-null MEFs were infected with retroviruses encoding Ras^V12 or empty vector, whole-cell extracts were prepared from 2 days till 6 days postinfection and were subjected to Western blot analysis with the indicated antibodies. (E) Wild-type MEFs were infected with the above-mentioned retroviruses, and total RNA was extracted at 2, 3, or 5 days postinfection. Mouse 47S pre-rRNA levels were analyzed by real-time PCR and graphed in a time-dependent manner. Changes in DHX33 protein levels in the time course were also graphed after quantitation of DHX33 signals in panels A to C after normalization to the empty vector control. Error bars were taken from three independent experiments.