Fig 2.

Reduction of DHX33 by ARF infection is dependent on p53 and Mdm2. (A) Wild-type MEFs, p53-null MEFs, p53^−/−; Mdm2^−/− MEFs, and p53^−/−; Mdm2^−/−; Arf^−/− MEFs were infected with retroviruses encoding either pBABE empty vector or pBABE-HA-ARF. Whole-cell lysates were prepared 5 days postinfection and were subjected to Western blot analysis by the indicated antibodies. (B) Quantitation of the DHX33 protein levels after normalization to empty vector in each group, error bars were taken from three independent experiments. (C) MEFs were infected by retrovirus encoding pBABE empty vector or pBABE-HA-ARF. At 4 days postinfection, cells were harvested and analyzed by Western blotting with the indicated antibodies. At 4 days postinfection, cells were also pulsed by [³H]uridine and chased at the indicated time points. Total RNA were analyzed for 47S pre-rRNA levels. (D) WT MEFs or p53-null MEFs were infected with lentivirus encoding either shSCR or shARF. At 4 days postinfection, the cells were harvested and subjected to Western blot analysis with the indicated antibodies.