Fig 6.

DHX33 protein induction is under translational control. (A) A total of 3 × 10⁶ Arf-null cells infected with retroviruses encoding empty vector or Ras^V12 at 4 days postinfection were subjected to cytosolic ribosome profiling. (B) The resultant fractionations from above were analyzed by RT-PCR for DHX33 mRNA distribution on ribosomes. GAPDH was used as a negative control. Bar data were taken from three independent experiments. (C) Ras^V12-infected Arf-null cells at 4 days postinfection were treated with rapamycin at 100 nM for 24 h. Whole-cell extracts were then subjected to total protein analysis by Western blotting with the indicated antibodies. The fold change is indicated underneath the blots. (D) A total of 3 × 10⁶ of Ras^V12-infected Arf-null cells after rapamycin treatment (100 nM) were subjected to cytosolic ribosome profiling. (E) The resultant fractions from above were analyzed by RT-PCR for DHX33 mRNA distribution on ribosomes. GAPDH was used as a negative control. The data represents a typical result from three independent experiments.