Skip to main content
. 2013 Apr;33(7):1303–1316. doi: 10.1128/MCB.00858-12

Fig 1.

Fig 1

Physiological concentrations of SCFA stimulate ANGPTL4 mRNA and protein secretion in colon adenocarcinoma cells. (A) Total SCFA concentration in different sections of the intestine of mice fed a low-fat diet. Errors bars represent SEM. (B) Concentration of individual SCFA in colon of mice fed a low-fat diet. Errors bars represent SEM. (C) ANGPTL4 concentration in medium of T84 and HT29 cells treated with SCFA for 24 h at the indicated concentrations. (D) ANGPTL4 mRNA in T84 and HT29 cells treated with SCFA for 24 h at the indicated concentrations. (E) Time course of induction of ANGPTL4 mRNA in T84 cells by butyrate (1 mM). (F) Inhibitory effect of α-amanitin (10 μg/ml) on induction of ANGPTL4 secretion by 1 mM butyrate in T84 cells. (G) Effect of rectal infusion of propionate on Angptl4 mRNA in epithelial scrapings of mouse colon. Errors bars represent SEM. (H) Time course of regulation of ANGPTL4 protein in medium of T84 and HT29 cells by trichostatin A (100 nM) and butyrate (8 mM). (I) Stimulatory effect of trichostatin A (100 nM) on LGALS1 mRNA in T84 cells. Error bars represent standard deviations (SD) except where indicated otherwise. An asterisk indicates a result significantly different from that of the control according to Student's t test (P < 0.05).