Fig 2.

PPARγ potently stimulates ANGPTL4 in colon adenocarcinoma cells. Synthetic agonists for PPARα (Wy14643 [WY]; 5 μM), PPARδ (GW501516 [GW]; 1 μM), and PPARγ (rosiglitazone [Rosi]; 1 μM) stimulate ANGPTL4 secretion (A) and mRNA expression (B) in T84 and HT29 cells. Cntl, control. (C) Amplification curve of PPARα, PPARδ, and PPARγ mRNA as determined by qPCR of T84 and HT29 cells. Sizes of amplicons varied less than 10%. (D) Time course of induction of ANGPTL4 protein in medium by rosiglitazone (1 μM). (E) Dose-dependent induction of ANGPTL4 protein in medium by rosiglitazone. Unless indicated otherwise, cells were treated for 24 h. Error bars represent SD. An asterisk indicates a result significantly different from that of the control according to Student's t test (P < 0.05).