Fig 3.

Induction of ANGPTL4 by butyrate is mediated by PPARγ. (A) Inhibitory effect of PPARγ antagonist GW9662 (5 μM) on induction of ANGPTL4 secretion in medium by rosiglitazone (10 nM) and butyrate (1 mM) in T84 cells. Effect of siRNA-mediated PPARγ knockdown (B, left) in T84 cells on butyrate-induced upregulation of ANGPTL4 mRNA (B, right) and ANGPTL4 protein in medium (C). (D) PPARγ ChIP-qPCR on selected loci in T84 cells treated with butyrate (8 mM) or rosiglitazone (1 μM) for 24 h. Bars represent the mean recovery plus ranges from two independent experiments. Error bars represent SD except when indicated otherwise. An asterisk indicates a significantly different result from that of the control according to Student's t test (P < 0.05).