Fig 4.

Global effects of butyrate in T84 cells. T84 cells were treated with rosiglitazone (1 μM) or butyrate (1 and 8 mM) for 24 h and subjected to Affymetrix microarray analysis. (A) Hierarchical clustering based on Pearson's correlation with average linkage. (B) Effect of siRNA-mediated PPARγ knockdown in T84 cells on induction of PLIN2 and UCP2 mRNA by butyrate. AQP8 mRNA (C) and PLIN2 mRNA (D) levels were determined in T84 cells treated with SCFA for 24 h at the indicated concentrations. Error bars represent SD. An asterisk indicates a result significantly different from that of the control according to Student's t test (P < 0.05).