Fig 3.

SCO2 induces expression of apoptotic genes in cancer cells. A PCR gene array for genes involved in the regulation of cellular apoptosis was conducted by using SCO2 cDNA-transfected p53 wild-type cells (KB, MCF-7, and HCT p53^+/+), p53 mutant cells (A-431), and p53 null cells (HCT p53^−/− and H1299). Overexpression of SCO2 resulted in the downregulation of genes inhibiting apoptosis and the upregulation of proapoptotic genes (lanes 13, 17, 19, 20, 23, and 24). ROS quenching in SCO2-overexpressing cell resulted in inhibition of apoptotic genes and increases in the expression levels of antiapoptotic genes (lanes 1 to 4, 6, and 8). Untreated cells were used as controls (lanes 5, 7, and 9 to 12). Tamoxifen was used as a positive control, which led to upregulation of genes involved in apoptosis (lanes 14 to 16, 18, 21, and 22) (n = 10).