Fig 4.

SCO2 induces expression of apoptotic genes in tumor xenografts. A PCR gene array for genes involved in regulation of cellular apoptosis was conducted by using SCO2 cDNA-transfected A-431, MCF-7, HCT p53^+/+, and HCT p53^−/− tumor xenografts. Overexpression of SCO2 resulted in the downregulation of genes inhibiting apoptosis and the upregulation of proapoptotic genes (lanes 1, 6, 7, and 9). ROS quenching in SCO2-overexpressing cell resulted in inhibition of apoptotic gens and increases in the expression levels of antiapoptotic genes (lanes 2 and 12 to 14). Untreated cells were used as controls (lanes 10, 11, 15, and 16). Tamoxifen treatment was used as a positive control, which led to the upregulation of genes involved in apoptosis (lanes 3 to 5 and 8) (n = 5).