Fig 1.

Subcellular distribution of Slap. Cell lysates from HEK293 cells transduced with a nonintegrating lentiviral vector expressing Slap and eGFP from a bicistronic mRNA were used to examine the specificity of a Slap polyclonal antibody. (a) Western blots reveal a major band of 34 kDa in cells transduced with the Slap-expressing vector that was absent from cells transduced with the control, eGFP-only lentiviral vector. (b and c) Immunolabeling for Slap shows that specific labeling is evident only in cells transduced with the Slap-expressing vector (b) and not with the control vector (c). (d and e) Representative images from dissociated 21DIV hippocampal neurons labeled for Syp and PSD95 (green in panels d and e, respectively) and Slap (red) show that Slap is localized at the postsynaptic terminal. A higher magnification image of the boxed area in panel d (below the main image) shows that Slap is juxtaposed to Syp labeling (d) and colocalizes with PSD95 (e). (f) Western blots of subcellular fractions from adult rat brain homogenate show that Slap is enriched at synaptic contacts. Bar, 10 μm.