Fig 2.

Phosphate deprivation leads to the downregulation of Cln3p. (A) Strain YAN32 (triple tagged: Cln2-HA, Clb5-TAP, and Sic1-Myc) was grown exponentially in synthetic complete medium. At time zero, the cells were harvested and incubated in phosphate (PO₄²⁻)-free medium. At the indicated times, samples were recovered and then analyzed for different proteins by immunoblotting using specific antibodies. (B) The wild-type strain was treated and sampled as described in Materials and Methods. Transcripts were analyzed using RT-PCR with specific primers. Data ± standard deviations from three independent experiments are shown. (C) Strains YPC702 (SWI6-TAP), YNR11 (WHI5-TAP), and YNR55 (CLN3-MYC) were treated, sampled, and analyzed as described for panel A.