Table 2.
Plasmids used in this study
| Name | Relevant characteristics | Reference or source |
|---|---|---|
| pCM64 | YCplac22-ADHp-CLN2 | 14 |
| pJC1065 | pEG(KG)-PHO85 (cloned in BamHI) | This study |
| pJC1437 | pEG(KG)-PHO80 (cloned in BamHI) | This study |
| pJC1280 | pRS416-CLN3-MYC | This study |
| pJC1161 | pGEX6P1-PHO80 (cloned in BamHI) | This study |
| pJC1164 | pGEX6P1-PHO85 (cloned in BamHI) | This study |
| pJC1154 | pGEX6P1-CLN3 (from nucleotide 1038 to stop codon) | This study |
| pJC1448 | pGEX6P1-CLN3 (from nucleotide 1038 to stop codon) with the 2 putative Pho85 sites (S449 and T520) mutated to Asp | This study |
| pJC1456 | pGEX6P1-CLN3 (from nucleotide 1038 to stop codon) with the 2 putative Pho85 sites (S449 and T520) mutated to Ala | This study |
| pJC1449 | pGEX6P1-CLN3 (from nucleotide 1159 to stop codon) with the 8 putative Cdc28 sites (T420, S455, S462, S466, S466, T478, S514, and T517) mutated to Ala | This study |