Table 1.
Primers used for constructing expression clones
| Gene | Vector | Orientationa | Primer sequence (5′–3′) |
|---|---|---|---|
| cnpv030 | pEFPlink2 | Fwd | CCGGATCCTCAGTCACGATGGATTTACC |
| cnpv030 | pEFPlink2 | Rev | CGATACTAGTTTATTTGTGTATATTTAAAGCG |
| fpv012 | pEFPlink2 | Fwd | CCGGATCCATGGATACAGAAATGGACGG |
| fpv012 | pEFPlink2 | Rev | GGTTCCACTAGTTTAACCAGTCTTATTATTAAC |
| fpv012 | pEFPlink2Flag | Fwd | CCGGATCCGATACAGAAATGGACGGTGTCAATAACG |
| fpv012mut1-126 (Mut1) | pEFPlink2Flag | Fwd | CCGGATCCGTAGATTCCTACACTCCGTTAC |
| fpv012mut1-222 (Mut2) | pEFPlink2Flag | Fwd | CCGGATCCACTAATGATGGTTATACGGCTC |
| fpv012mut1-324 (Mut3) | pEFPlink2Flag | Fwd | CCGGATCCAAATACGGTATTACTCCTCTTG |
| fpv012 | pEFPlink2Flag | Rev | CGATACTAGTTTAACCAGTCTTATTATTAACTTTAATAGCG |
| fpv012mut955-996 (Mut4) | pEFPlink2Flag | Rev | CGATACTAGTTTACAATTTTTTTATATTATCGTTATTAAG |
| fpv155 | pEFPlink2Flag | Fwd | CCGGATCCATGTTTAATAGTATGATAACCGG |
| fpv155 | pEFPlink2Flag | Rev | CGATACTAGTTTAGTTATCTGAAAATATTTTATTTATATCC |
| fpv213 | pEFPlink2Flag | Fwd | GCATCCATGGGGGAACGAGTAAAAAAATGTTTTAG |
| fpv213 | pEFPlink2Flag | Rev | CGATGGATCCTTAGCTATCCTGTAAAGGAGATAC |
a
Fwd, forward; Rev, reverse.