. 2013 May;87(9):4808–4817. doi: 10.1128/JVI.00112-13

Table 1.

Mutation analysis of MMTV reverse transcription products^a

Genotype and temp (°C)	No. of mutations		No. of clones	Total no. of bp	G-to-A mutation frequency
Genotype and temp (°C)	G to A	Other	No. of clones	Total no. of bp	G-to-A mutation frequency
C57BL/6, 95	28	20	50	13,236	0.21
C57BL/6, 81–83	73	24	44	15,652	0.47
BALB/c, 95	2	2	18	4,050	0.05
BALB/c, 81–83	52	15	34	12,690	0.41
APOBEC3^−/−, 95	2	7	17	4,726	0.04
APOBEC3^−/−, 81–83	7	9	30	9,720	0.07

Sequences cloned from 3DPCR amplification products in Fig. 3A and B were aligned to their respective reference sequence. Rate of mutation (per 100 bp) for each condition was calculated for each of 3 to 5 sequencing runs. Unpaired, two-tailed t tests were performed using the mean values for each run. Sequences from C57BL6 viral DNA isolated using a low denaturing temperature contained significantly more mutations than APOBEC3^−/−-isolated DNA (P < 0.05) but not significantly more mutations than BALB-isolated viral DNA (P = 0.67). Deamination levels in viral DNA isolated from all three genotypes using a regular denaturing temperature were not statistically significantly different from one another.