Table 2.
Mutation spectra in the HSA mutation target gene of HIV-1 proviral sequencesa
| Mutation | % of cells with mutation |
|||
|---|---|---|---|---|
| CEM-GFPb | U373-MAGIc | 293Td | SupT1e | |
| G-to-A | 29 | 26 | 17 | 17 |
| A-to-G | 27 | 29 | 29 | 29 |
| T-to-C | 27 | 29 | 30 | 36 |
| C-to-T | 10 | 10 | 14 | 11 |
a
Cells were transduced with an HIV-1 vector (HIG) pseudotyped with vesicular stomatitis virus protein G (VSVG), and the HSA reporter gene sequence from the provirus in infected cells was PCR amplified and sequenced. The percentage of mutations for each mutation type compared to the total mutations identified is indicated.
b
Total no. of sequences, 238; total no. of mutations, 365.
c
Total no. of sequences, 225; total no. of mutations, 329.
d
Total no. of sequences, 154; total no. of mutations, 199.
e
Total no. of sequences, 173; total no. of mutations, 219.