Table 3.
Target cells | No. (%) of mutant sequences with multiple G-to-A mutations/total mutants analyzed |
---|---|
CEM-GFP | 14/238 (5.9) |
U373-MAGI | 10/225 (4.4) |
293T | 2/154 (1.3) |
SupT1 | 0/173 (0) |
Cells were transduced with an HIV-1 vector (HIG) pseudotyped with vesicular stomatitis virus protein G (VSVG), and the HSA reporter gene sequence from the provirus in infected cells was PCR amplified and sequenced. The number of proviral sequences having HSA reporter gene sequences with multiple G-to-A mutations from each cell type was divided by the total number of proviral sequences identified with mutations.