Fig 3.

Dual vaccination with VRP-SUDV GP and VRP-EBOV GP protects cynomolgus macaques from SUDV i.m. challenge. NHPs were vaccinated with VRP expressing irrelevant antigen (n = 1) or VRPs expressing both SUDV GP and EBOV GP (n = 3). SUDV GP (A) or EBOV GP (B) specific serum antibody end titers were determined by ELISA at the indicated times postvaccination or challenge. Dotted line indicates assay limit of detection. Black and red arrows indicate time of SUDV challenge and EBOV back-challenge, respectively. (C) NHPs were challenged by i.m. injection of 1,000 PFU of SUDV 28 days postvaccination. (D) Surviving NHPs were then back challenged by i.m. injection of 1,000 PFU of EBOV 30 days post-SUDV challenge. **, P ≤ 0.01 (as determined by the Fisher exact test).