Table 3.
Individual | Age at sampling (yr) | Infection status | No. of virus strains | No. of virus strains transmitted | Time between samplings (yr) | Virus yr based on no. of strains and time between samplings | Virus stability (yr) |
---|---|---|---|---|---|---|---|
Gogol | 10 | Single infection | |||||
Gogol | 17 | Single infection | 1 | 1 | 7 | 7 | 7 |
Kabisha | 24 | Superinfection | |||||
Kabisha | 25 | Superinfection | 2 | 2 | 1 | 2 | 2 |
Kinshasa | 11 | Single infection | |||||
Kinshasa | 17 | Single infection | 1 | 1 | 6 | 6 | 6 |
Rubra | 31 | Single infection | |||||
Rubra | 35 | Superinfection | 1 | 1 | 4 | 4 | 4 |
Rubra | 38 | Superinfection | 2 | 2 | 3 | 6 | 6 |
Sagu | 13 | Single infection | |||||
Sagu | 17 | Superinfection | 1 | 1 | 4 | 4 | 4 |
Sagu | 19 | Superinfection | 2 | 1 | 2 | 4 | 2 |
Sumatra | 38 | Single infection | |||||
Sumatra | 40 | Superinfection | 1 | 1 | 2 | 2 | 2 |
Utan | 7 | Single infection | |||||
Utan | 11 | Single infection | 1 | 1 | 4 | 4 | 4 |
Yucca | 32 | Superinfection | |||||
Yucca | 34 | Superinfection | 2 | 2 | 2 | 4 | 4 |
Zyon | 37 | Superinfection | |||||
Zyon | 42 | Superinfection | 2 | 2 | 5 | 10 | 10 |
Zyon | 44 | Superinfection | 2 | 2 | 2 | 4 | 4 |
Total | 42 | 57 | 55 | ||||
Persistence (%) | 96 |
Individuals for which multiple samples were available were tested for virus stability. Strains identified in the earlier samples were compared with strains found in the later corresponding sample. A virus strain was defined using network-based analysis [identification of the founder sequence(s)/group of closely related sequence in the absence of the founder sequence]. Strains were defined as stable in the consecutive sample(s) if sequences revealed a maximal distance of 0.7% observed divergence (up to 3 bp difference). Of note, estimation of the number of independent infection events is a minimum estimate.