Fig 5.

(A) The 5′ UTR is not a major determinant of the defectiveness of pKoRV-Cindy. Infectious molecular clones were transfected into HEK293T(LacZ) cells, and virus production was monitored by the LacZ marker rescue assay. (B) Host ranges of KoRV pseudotype virus. lacZ(KoRV522) was inoculated onto the indicated cell lines, and the virus titers were determined by the LacZ assay. (C) Comparison of viral titers of pseudotype viruses bearing KoRV, GALV, and FeLV-B Envs. Each pseudotype virus was inoculated onto naïve HEK293T cells, and the virus titer was determined by the LacZ assay. The data were obtained by three independent experiments, and the values are expressed as the mean ± 1 standard deviation (SD).