Fig 6.

MHC class I presentation of IE1-derived peptides at IE times after infection with mutants expressing EYFP-pp71. HFF were infected with the indicated viruses at 125 genomes per cell in the presence of 250 μg/ml CX. At 9 h after infection, culture medium was replaced by medium containing 10 μg/ml AcD. Incubation of infected cells was continued for another 13.5 h. The cells were then fixed with 0.5% paraformaldehyde and subjected to IFN-γ ELISpot analysis, using IE1_TMY-specific CD8 T cells as responders. The results for three wells (white and gray circles) (each symbol represents the value for one well) and the mean values (horizontal lines) are shown.