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Formation of the aggresome structure is not conserved among serotypes. H1299 cells were transfected with plasmid DNAs expressing HA-tagged E1B55K of different serotypes. (A) Transfected cells were fixed in methanol and stained for HA, γ-tubulin, and DNA (with DAPI). (B) Transfected cells were treated for the last 6 h with nocodazole, fixed in paraformaldehyde, and stained for HA and DNA (with DAPI). Panels are as indicated in the figure.
