Table 2.

The NP-blocking ELISA on pre-HPAI H5N1 challenge serum showed all LAIV-vaccinated animals were positive for NP antibodies^a

Group	Animal no.	NP blocking ELISA S/N ratio	Result
H9N2-GFP	709	0.141	+
	710	0.169	+
	711	0.134	+
	712	0.169	+
	713	0.180	+
	714	0.190	+
H9N2-H5	715	0.171	+
	716	0.191	+
	718	0.124	+
	719	0.143	+
	720	0.138	+
PBS	721	0.651	−
	722	0.646	−
	723	0.727	−
ΔH5N1	724	0.141	+
	725	0.148	+
	726	0.131	+

^aSerum was obtained from animals 13 days post-boost vaccination. NP ELISA titers were determined by using the Flu Detect BE kit (avian influenza virus antibody test kit; cELISA) from Synbiotics (College Park, MD), following the manufacturer's directions. A signal-to-noise (S/N) ratio of less than 0.6 was considered positive for antibody against influenza virus.