Fig 2.

Viral protein expression in JB-replicating Huh7.5 cells. Naïve Huh7.5 cells were infected with JFH1 virus, and immunofluorescence (IF) staining was performed to detect viral structural proteins. Naïve Huh7.5-NS5A cells were infected with JB virus, stable viral replication was selected by blasticidin, and IF staining was performed to detect viral structural proteins. (A) Schematic representation of JB viral genome containing BSD resistance gene insertion in the C-terminal domain of NS5A at position 2395 (in polyprotein amino acid sequence). (B) Immunofluorescent detection of HCV core (red) and gpE2 (green) proteins with lipid droplet stain (blue). Scale bar, 5 μm. (C) Immunofluorescent detection of HCV core (red) and gpE2 (green) proteins with nucleus counterstain (blue). Scale bar, 25 μm.