Fig 5.

Modulation of HCV RNA replication in JB-replicating hepatoma cell lines. Huh7.5-NS5A-JB (A) or HepG2c-NS5A-JB (B) cells were treated with increasing concentrations of drugs for 24 h, and HCV RNA replication was assessed by one-step quantitative RT-PCR on cellular RNAs. Results were normalized with total RNA concentration and glucuronidase gene expression. Data are presented as fold increase compared to untreated cells. Error bars represent standard deviations of two independent experiments. Ethanol (EtOH), 0, 0.5, 1, and 2%; chenodeoxycholic acid (CDCA), 0, 25, 50 and 100 mM; U0126, 0, 2.5, 5 and 10 μM; oleic acid (OA), 10% (vol/vol). (C) HepG2c-JB cells (without V5-tagged NS5A) were transcomplemented with NS5A using a lentiviral vector. Six days after lentiviral transduction, intracellular HCV RNA was quantified by quantitative RT-PCR in transcomplemented and control cells. (D) At the same time, extracellular HCV RNA was also quantified by quantitative RT-PCR.