Table 1.
Generation of HCV-replicating Huh7.5 and HepG2 cell lines
| Naïve cell line | No. of BSD-resistant coloniesa | BSD-resistant cell lineb |
|---|---|---|
| Huh7.5 | >10,000 | Yes |
| Huh7.5-NS5A | >10,000 | Yes |
| HepG2-CD81 | Transient survivalc | No |
| HepG2-CD81-shINFβ-shINFR1 | 25 | No |
| HepG2c-CD81 | 500 | Yes |
| HepG2c-CD81-NS5A | 300 | Yes |
| HepG2c-CD81-IRF3(ΔN) | 800 | Yes |
| HepG2c-CD81-IRF3(ΔN)-NS5A | 800 | Yes |
| Differentiated HepaRG cells | Transient survivalc | No |
| PHH | Transient survivalc | No |
a
Approximate number of BSD-resistant colonies (extrapolated from the highest positive viral dilution).
b
Successful establishment of a BSD-resistant polyclonal cell line.
c
Transient survival to BSD for up to 1 week, followed by cell death without stable resistance.