Fig 5.

Nucleozin does not disrupt the main exocytic pathway. (A to C, E, F) A549 cells were infected with the WT virus, and at 6 hpi, 1 μM nucleozin (NCZ) was added to one set. Samples were fixed at 8 hpi and stained for NP and calnexin (A); NP and GM130 (B); NP and clathrin, EEA1, or LAMP1 in the presence of nucleozin (C); M2 (E); HA (PR8) and NA (F); or NP and α-tubulin (α-tub) (G). Merged images include a DAPI channel shown in blue. Scale bars, 10 μm unless indicated otherwise. In panel D, lysates from cells treated and harvested as indicated were analyzed by Western blotting for HA, M2, and (as a loading control) α-tubulin (α-tub). Images are representative of three independent experiments.