Fig 4.

ASC and caspase-1 interact and redistribute in the cytoplasm of cells latently infected with KSHV. TIVE and TIVE-LTC cells fixed with 2% paraformaldehyde (A and B) or BJAB and BCBL-1 cells fixed with ice-cold acetone (C and D) were subjected to immunofluorescence analysis. Cells were stained with anti-ASC and anti-caspase-1 antibodies and visualized by incubation with Alexa Fluor 488 (green) and Alexa Fluor 594 (red) secondary antibodies, respectively. Cell nuclei were stained with DAPI (blue). The boxed areas were enlarged and are shown in the rightmost panels. White and red arrows, colocalization of ASC and caspase-1 in the cytoplasm and nucleus, respectively. Image results are depicted from a representative field taken after three independent experiments were performed. Magnification, ×60.